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ASCE期刊回复意见的投稿须知

发布时间:2024-07-03 03:15:31

ASCE期刊回复意见的投稿须知

ACS Sensors是SCI Chemical Engineering类1区期刊,投稿难度大。 ACS SENSORS期刊ISSN2379-3694。自引率4.90%。 ACS SENSORS期刊官方网站期刊ACS SENSORS投稿网址 涉及的研究方向Chemical Engineering-Bioengineering,出版国家或地区UNITED STATES,出版语言English。 期刊SCI分区 学科分区CHEMISTRY, ANALYTICALQ1CHEMISTRY, MULTIDISCIPLINARYQ1NANOSCIENCE & NANOTECHNOLOGYQ2

ASCE是三区SCI首先,sci几区按jcr划分jcr分区根据影响因子(IF值),某一个学科的所有期刊都按照上一年的影响因子降序排列,然后平均4等分(各25%),分别是Q1,Q2,Q3,Q4。其次,sci几区按中科院划分一区刊:各类期刊三年平均影响因子的前5%,二区刊:前6%-20%,三区刊:前21% - 50%,四区刊:后51%-100%。sci几区不管是jcr分区还是中科院分区,都是把收录在sci中的期刊根据一定的标准划分了四个分区,且划分标准都与影响因子有关。但采用影响因子的基数以及期刊数量占比是不同的。jcr分区是上一年影响因子,把sci期刊平均分配,中科院分区是三年平均影响因子,把sci期刊按不同的比例分配,呈现金字塔形状。sci几区中,影响力从高到低依次是一区、二区、三区和四区,投稿一区的sci论文,含金量最高,难度也最大。好的文章,可以挑战高分区,而相对一般的文章,考虑低分区,即作者要根据文章的实际情况量力而行。另外,sci期刊录用文章起点是很高的,若连四区的sci都不达标,那说明文章还达不到发表sci的标准。作者要不提高写作文章的质量,要不找更低级别的期刊投稿发表。

期刊投稿回复意见

1、仔细阅读审稿人意见,确保自己明白问题所在及为什么审稿人会提出这些问题。当稿件被拒时,您可能会沮丧或者对所提问题持不同意见。但是,被拒和不同意见是科研分析和辩论的正常部分,所以不要认为他们是针对您个人的。请记住他们只是想帮您改进论文。如果您不明白某个意见,应在您的答复中明确提出来,或者给编辑写信要求进一步解释。但是,在此之前,请与您的合作作者讨论一下,确保你们都清楚审稿人意见并确定后续如何处理这些意见。2、准备回复文件。考虑每条意见以及如何答复。写答复时,应做到清楚、简明扼要,并用证据说明您做出的改动或者您采用特定的方式处理意见的原因。3、逐一答复每条意见和修改建议。• 将每条意见复制、粘贴到一个新文件中(不要只提“审稿人1,意见1”,因为这需要期刊工作人员花时间相互参照)。请在每条意见后写答复。• 提到您对哪些地方做出了修改。不要只提及页码,而是使用行号,或者引用某个特定部分的句子开头部分。• 明确解释您对哪些建议持反对意见及理由(并且给出您的证据)。• 务必做到积极、礼貌和简明扼要。4、不要错过截止期限!大多数期刊都有接收修回稿的具体期限。确保自己按时答复!计划好答复时间,以便您有足够的时间撰写答复,并对文章做出修改。如果您需要重新查询数据或者做出大范围修改,比如减少字数或者增加额外内容,您可能需要花费一定的时间。5、不要向其他期刊提交原稿。即使您的论文被完全拒绝,也不要将原稿直接发给另一家期刊,可能别的期刊也会给出类似意见(甚至可能使用同样的审稿人!),因此您应根据反馈意见修改论文,然后确定后续步骤。如果审稿人意见不一致怎么办?这种情况其实很普遍。例如,审稿人1可能说“表2多余—请删除”,而审稿人2可能会说“表2的第3列数据集还需进一步解释。”您该接受哪个意见?自己做出判断,并征求合作作者的意见,在答复中明确说明您为何选择删除数据而不是增加数据。再次强调,在提及您做出的修改时务必明确—引用具体行数/表号,您做出了哪些修改以及原因。……如果我的论文还是被拒了怎么办呢?在这种情况下,和您的合作作者讨论如何回应,决定是否向期刊编辑申诉……还是转而向其他期刊投稿。如上所述,在未充分考虑其他期刊的性质和范围之前,不要贸然将原稿投给它们。 以上内容来自查尔斯沃思论文小贴士,希望可以帮助您,谢谢

1.所有问题必须逐条回答。 2.尽量满足意见中需要补充的实验。3.满足不了的也不要回避,说明不能做的合理理由。 4.审稿人推荐的文献一定要引用,并讨论透彻。 以下是本人对审稿人意见的回复一例,仅供参考。续两点经验:1. 最重要的是逐条回答,即使你答不了,也要老实交代;不要太狡猾,以至于耽误事;2. 绝大部分实验是不要真追加的,除非你受到启发,而想改投另外高档杂志----因为你既然已经写成文章,从逻辑上肯定是一个完整的 “story” 了。以上指国际杂志修稿。国内杂志太多,以至于稿源吃紧,基本没有退稿,所以你怎么修都是接受。我的文章水平都不高,主要是没有明显的创新性,也很苦恼。但是除了开始几篇投在国内杂志外,其他都在国际杂志(也都是SCI)发表。以我了解的情况,我单位其他同志给国内杂志投稿,退稿的极少,只有一次被《某某科学进展》拒绝。究其原因,除了我上面说的,另外可能是我单位写稿子还是比较严肃,导师把关也比较严的缘故。自我感觉总结(不一定对):1)国内杂志审稿极慢(少数除外),但现在也有加快趋势;2)国内杂志编辑人员认真负责的人不多,稿子寄去后,少则几个月,多则一年多没有任何消息;3)国内杂志要求修改的稿子,如果你自己不修,他最后也给你发;4)国外杂志要求补充实验的,我均以解释而过关,原因见少帖)。还因为:很少杂志编辑把你的修改稿再寄给当初审稿人的,除非审稿人特别请求。编辑不一定懂你的东西,他只是看到你认真修改,回答疑问了,也就接受了(当然高档杂志可能不是这样,我的经验只限定一般杂志(影响因子1-5)。欢迎大家批评指正。我常用的回复格式:Dear reviewer:I am very grateful to your comments for the manuscript. According with your advice, we amended the relevant part in manuscript. Some of your questions were answered below.1)2)....引用审稿人推荐的文献的确是很重要的,要想办法和自己的文章有机地结合起来。至于实验大部分都可以不用补做,关键是你要让审稿人明白你的文章的重点是什么,这个实验对你要强调的重点内容不是很必要,或者你现在所用的方法已经可以达到目的就行了。最后要注意,审稿人也会犯错误,不仅仅是笔误也有专业知识上的错误,因为编辑找的审稿人未必是你这个领域的专家。只要自己是正确的就要坚持。在回复中委婉地表达一下你的意见,不过要注意商讨语气哦!我得回复格式是这样的:Dear Professor xx:Thank you very much for your letter dated xxx xx xxxx, and the referees’ reports. Based on your comment and request, we have made extensive modification on the original manuscript. Here, we attached revised manuscript in the formats of both PDF and MS word, for your approval. A document answering every question from the referees was also summarized and enclosed.A revised manuscript with the correction sections red marked was attached as the supplemental material and for easy check/editing purpose.Should you have any questions, please contact us without hesitate.然后再附上Q/A,基本上嘱条回答,写的越多越好(老师语)。结果修改一次就接收了:)我的回复,请老外帮忙修改了Dear Editor:Thank you for your kind letter of “......” on November **, 2005. We revised the manuscript in accordance with the reviewers’ comments, and carefully proof-read the manuscript to minimize typographical, grammatical, and bibliographical errors.Here below is our description on revision according to the reviewers’ comments.Part A (Reviewer 1)1. The reviewer’s comment: ......The authors’ Answer: .....2. The reviewer’s comment: ......The authors’ Answer: ...........Part B (Reviewer 2)1. The reviewer’s comment: ......The authors’ Answer: .....2. The reviewer’s comment: ......The authors’ Answer: ...........Many grammatical or typographical errors have been revised.All the lines and pages indicated above are in the revised manuscript.Thank you and all the reviewers for the kind advice.Sincerely yours,***一个回复的例子(已接收)Major comments: 1. The authors need to strengthen their results by including MMP secretion, and tran-matrigel migration by a positive control progenitor cell population i.e. enriched human CD34 cells obtained from mobilized PBL, since this is a more clinically relevant source of CD34 cells which has also been shown to secrete both MMP-9 and MMP-2 (ref. 11). CD34 enriched cells from steady state peripheral blood which also secrete MMPs are also of interest. 2. In fig 1C please specify which cell line represents MMP-negative cells. This needs to be clarified, as well as a better explanation of the method of the protocol. 3. The ELISA results are represented as "fold increase" compared to control. Instead, we suggest that standards should be used and results should be presented as absolute concentrations and only then can these results be compared to those of the zymography. 4. When discussing the results, the authors should distinguish clearly between spontaneous migration vs chemotactic migration. Furthermore, the high spontaneous migration obtained with cord blood CD34 cells should be compared to mobilized PBL CD34 enriched cells and discussed. 5. The authors claim that the clonogenic assay was performed to determine the optimum concentration for inhibition of MMP activity by phenanthroline and anti MMP-9 mAb, however they should clarify that this assay can only determine the toxicity of the inhibitors and not their optimal inhibitory concentrations.Minor comments: 1. There are many spelling and syntax errors, especially in the results and discussion, which need correction. a. Of special importance, is the percent inhibition of migration, which is described as percent of migration. i.e. pg 7:"Migration of CB CD34 was reduced to 73.3%?" Instead should read "Migration of CB CD34 was reduced by 73.3%?" b. The degree symbol needs to be added to the numbers in Materials and methods. 2. It would be preferable to combine figure 1A and B, in order to confirm the reliability of fig. 1B by a positive control (HT1080).Answer to referee 1 comment: 1. Mobilized peripheral blood is a more clinical source of CD34+ cells, so it is necessary to compare the MMP-9 secretion and trans-migration ability of CB CD34+ cells with that of mobilized PB CD34+ cells. However, we couldn't obtain enough mobilized PB to separate PB CD34+ cells and determine the MMP-9 secretion and migration ability, so we couldn’t complement the study on PB CD34+ cells in this paper. Results obtained by Janowska-Wieczorek et al found that mobilized CD34+ cells in peripheral blood express MMP-9. Furthermore, Domenech’s study showed that MMP-9 secretion is involved in G-CSF induced HPC mobilization. Their conclusions have been added in the discussion. In our present study, our central conclusion from our data is that freshly isolated CD34+ stem/progenitor cells obtained from CB produce MMP-9. 2. MMP-9 negative cell used in fig 1C was Jurkat cell. In zymographic analysis, MMP-9 was not detected in the medium conditioned by Jurkat cell. To exclude that the contaminating cells may play a role in the observed MMP-9 production, we screened the media conditioned by different proportion of CB mononuclear cells with MMP-9 negative cells by zymography. This result may be confusion. Actually, only by detecting the medium conditioned by 2X105 CB mononuclear cells (MNC)/ml (since the purities of CD34+ cell are more than 90%), it could exclude the MNC role. In the revised manuscript, we only detected MMP-9 activity and antigen level in the medium conditioned by 2X105 CB mononuclear cells (MNC)/ml. There is no MMP-9 secretion be detected in the medium conditioned by 2X105 CB MNC/ml. It excluded the possibility that the MMP-9 activity in CB CD34+ cells conditioned medium is due to the contamination by MNC. 3.In this revised paper, we have detected the MMP-9 antigen levels by using commercial specific ELISA kits (R&D System, sensitivity, 0.156ng/ml). Recombinant MMP-9 from R&D System was used as a standard. The results are expressed in the absolute concentration. The absolute concentration result has been added in the paper. As shown in Fig2, MMP-9 levels were detectable in both CB CD34+ cell conditioned medium and BM CD34+ cell conditioned medium. However, MMP-9 level was significantly higher in CB CD34+ cell conditioned medium than in BM CD34+ cell conditioned medium (0.406±0.133ng/ml versus 0.195±0.023ng/ml). Although gelatinolytic activity was not detected in media conditioned by CD34+ cells from BM, sensitivity of ELISA favors the detection of MMP-9 antigen in the BM CD34+. 4. In our study, to establish the direct link between MMP-9 and CB CD34+ cells migration, we only determined the role of MMP-9 in spontaneous migration of CB CD34+ cells, but not in chemotactic migration. Actually, regulation of hematopoietic stem cell migration, homing and anchorage of repopulation cells to the bone marrow involves a complex interplay between adhesion molecules, chemokines, cytokines and proteolytic enzymes. Results obtained by the groups of Voermans reveal that not only the spontaneous migration but also the SDF-1 induced migration of CB CD34+ cells is greatly increased in comparison to CD34+ cells from BM and peripheral blood. 5. CD34+ cells we obtained in each cord blood sample were very limited. It is not enough to screen the inhibitors concentrations to select the optimal inhibitory concentrations. In the blocking experiments, based on the concentrations used by others and the manufacturer's recommendation, we then determined the inhibitors concentrations by excluding the toxicity of the inhibitors in that concentration, which was determined by clonogenic assay.Minor comments:1.The spelling and syntax errors have been checked and corrected.2.Since the results in figure 1A and B were obtained from two separated and parallel experiments, it is not fitness to combine two figures.

具体问题具体回复,SCI常见的问题优助医学来说一下。编辑们一般会在decisionletter中阐述清楚他们是否认为稿件经修改后足够发表。如果你不确定编辑在信里说的是什么意见,那么不妨回信询问。编辑的工作就是与读者沟通,说明怎样的修改会使文章足够发表(如果他们认为该文章有机会发表到该期刊上),或者为什么作者的文章无法发表(如果他们认为文章没有机会发表)。如果编辑或审稿人认为你的文章当前无法发表,他们其实会指出了你的研究中还有什么需要补充的(或者如果你的文章是理论性的,还有哪些问题你需要阐述得更清楚)。如果你是向实证性期刊投稿,那么只要你增加了足够的数据,便可以再次投稿。当然,当你这样做的时候,最好直接能解决decision letter和审稿意见中的建议和问题。在大多数情况下,写一封Cover Letter来说明之前decision letter和审稿意见中提出了哪些问题,并说明新修改的稿件是如何解决这些问题的,会很有帮助(或者如果你没有解决这些问题,阐述清楚为什么这些问题不是阻碍文章发表的原因)。

asce期刊投稿须知

如果你确认被录用了,那么在出刊之后,杂志社会寄样刊给你的

这个状态很正常,但并不代表reviewer已经接受稿件了。

Under review表示审稿人的意见已上传,说明审稿人已接受审稿,正在审稿中,这应该是一个漫长的等待(期刊通常会限定审稿人审稿时间,一般为一个月左右)。

当然前面各步骤也可能很慢的,要看编辑的处理情况。如果被邀请审稿人不想审,就会decline,编辑会重新邀请别的审稿人。

ACS出版的36种纸本期刊,其中36种有电子版,这些期刊涵盖了24个主要的学科领域:

1.生化研究方法、药物化学、有机化学。

2.普通化学、环境科学、材料学、植物学。

3.毒物学、食品科学、物理化学、环境工程学。

4.工程化学、应用化学、分子生物化学、分析化学。

5.无机与原子能化学、资料系统计算机科学、学科应用。

6.科学训练、燃料与能源、药理与制药学。

7.微生物应用生物科技、聚合物、农业学。

期刊投稿回复意见后

首先,诚恳的态度是至关重要的,提交文章修改后要附上一个cover letter。里面包含这些内容:

虽然cover letter的内容也都是客套话,但是编辑跟审稿人看着也会舒心不少。特别是审稿人,需要认真地无偿地审阅文章,难能可贵的是还需要找出不足的地方。即便有时因为研究方向不是很一致,他们有的问题有点业余,又或者提意见时比较不客气,回复审稿意见的时候也一定要尊重他们。

第二,另外起草一个单独的response letter。 在这里用问答式一一列出每个审稿人的意见并且一一作答。对于文字的修改要求,直接接受就行了。有的审稿人要求增加参考文献,也许这是审稿人唯一显示他私心的地方——比如要求引用他的文章,不是很离谱的情况下也可以照办,或者打个折嘛,要求引用三篇最后加上一篇嘛。回答问题的时候,最好简洁和就事论事,不要拖泥带水。要注意不要为了回答某个问题而导致更多的疑问,尽量将讨论局限在有限的范围内。

第三,有的审稿人与文章的研究方向有差异,或者没有认真读文章,导致对文章的理解有误,从而提出一些莫名其妙的问题。回答这些问题的时候,可以首先引用一下文章的相关句子,然后指出文章的真正意思。接着承认是自己的表达出现问题了,让审稿人曲解了意思,最后指出句子已经重写,表达的意思已经更准确了。这样的回答,既巧妙地回答了该问题,也避免了让审稿人尴尬。

第四,如果遇到了非常难回答的问题,比如审稿人质疑文章的创新性有限,价值不大。这些是文章的硬伤,是没有办法修改的。赞同审稿人的意见肯定不好,但是用回避的方式不回答更不好,既不礼貌也侧面赞同了审稿人。这个问题尽管很难回答,但是还是要去争取一下,比如再强调一下文章里面相关的几个句子。要知道每个人的见解不同,虽然一个审稿人觉得意义不大,但是决定权毕竟是在编辑手里,只要编辑在综合多个审稿人意见之后还觉得文章有可取之处,也就没有问题。而response letter是所有审稿人都可以看到的,诚恳的回答会获得其他审稿人的好感。第五,审稿意见里面经常出现的问题是要求补充信息,比如更多的实验结果或者与该文章相关的另外的一些信息。这样的问题需要仔细斟酌一下,如果仅仅是审稿人出于自己的好奇,是可以选择在response letter 里提供而不是直接添加到文章里面。而如果对所有读者都有用,则需要加到文章里面。对于审稿人提出的不合理的建议,可以心平气和地找个客观的理由委婉地拒绝或者提供一些参考资料,不要让审稿人觉得你对他的问题视而不见。

要回复。1、职场礼貌别忽略,在工作之中,人们普遍采用的都是用各种社交软件或者说是一些办公软件,直接在上面进行工作上的沟通,和发送各种文件。在这个环节之中,少了人与人之间的直接交流,如果是人与人之间的直接交流,人们能够通过自己的肢体语言,脸部表情语气等等去表达自己,对对方的一种尊重以及礼貌。2、细节方面更要做好,给杂志社留下好印象。一个人对于其他人的印象,其实就是由这个人表现出来的这些林林总总的小细节所构成的。继续回复领导的话,这是一个小细节,这种小细节自己更要表现得好,争取一切的机会去给领导留下各种好印象。

教你搞定编辑处理信和审稿人意见的回复向期刊投稿只是论文发表过程的第一步。绝大多数情况下,论文都要根据期刊编辑和审稿人反馈意见经过一些改动或者修改,期刊直接接受作者文稿而且无需修改的情况极为罕见。每位作者在其职业生涯中都会面对稿件被拒的情况!首先,我们看看期刊常用的处理类型。尽管名称各不相同,但如下几种最为典型:1、不加评论完全拒绝2、提出意见后完全拒绝3、接受,但需要大修4、接受,但需要小修5、完全接受现在,让我们把精力放在第3种和第4种上,因为在这些情况下,论文还有被编辑接受的机会,但处理期刊审稿人的意见是确保论文被再次审核的关键一步,也是论文被编辑接受的最好机会。如何处理审稿人的意见?首先,期刊一般会将每个审稿人的意见做个完整清单,加上编辑处理信一起发给您。因此,您可以清楚地看到每位审稿人的意见。1、仔细阅读审稿人意见,确保自己明白问题所在及为什么审稿人会提出这些问题。当稿件被拒时,您可能会沮丧或者对所提问题持不同意见。但是,被拒和不同意见是科研分析和辩论的正常部分,所以不要认为他们是针对您个人的。请记住他们只是想帮您改进论文。如果您不明白某个意见,应在您的答复中明确提出来,或者给编辑写信要求进一步解释。但是,在此之前,请与您的合作作者讨论一下,确保你们都清楚审稿人意见并确定后续如何处理这些意见。2、准备回复文件。考虑每条意见以及如何答复。写答复时,应做到清楚、简明扼要,并用证据说明您做出的改动或者您采用特定的方式处理意见的原因。3、逐一答复每条意见和修改建议。• 将每条意见复制、粘贴到一个新文件中(不要只提“审稿人1,意见1”,因为这需要期刊工作人员花时间相互参照)。请在每条意见后写答复。• 提到您对哪些地方做出了修改。不要只提及页码,而是使用行号,或者引用某个特定部分的句子开头部分。• 明确解释您对哪些建议持反对意见及理由(并且给出您的证据)。• 务必做到积极、礼貌和简明扼要。4、不要错过截止期限!大多数期刊都有接收修回稿的具体期限。确保自己按时答复!计划好答复时间,以便您有足够的时间撰写答复,并对文章做出修改。如果您需要重新查询数据或者做出大范围修改,比如减少字数或者增加额外内容,您可能需要花费一定的时间。5、不要向其他期刊提交原稿。即使您的论文被完全拒绝,也不要将原稿直接发给另一家期刊,可能别的期刊也会给出类似意见(甚至可能使用同样的审稿人!),因此您应根据反馈意见修改论文,然后确定后续步骤。如果审稿人意见不一致怎么办?这种情况其实很普遍。例如,审稿人1可能说“表2多余—请删除”,而审稿人2可能会说“表2的第3列数据集还需进一步解释。”您该接受哪个意见?自己做出判断,并征求合作作者的意见,在答复中明确说明您为何选择删除数据而不是增加数据。再次强调,在提及您做出的修改时务必明确—引用具体行数/表号,您做出了哪些修改以及原因。……如果我的论文还是被拒了怎么办呢?在这种情况下,和您的合作作者讨论如何回应,决定是否向期刊编辑申诉……还是转而向其他期刊投稿。如上所述,在未充分考虑其他期刊的性质和范围之前,不要贸然将原稿投给它们。 参考:查尔斯沃思论文润色贴士

投稿期刊回复审稿意见

在回复审稿人意见的时候,诚恳的态度非常关键,最好可以附上一个新的cover letter。当你不同意审稿人的意见时,你可以说“Thank you for your comments. We understand your concern about XXX. However, because YYY. To make this clearer we have changed ZZZ on page/line XX.”首先站在审稿人的角度上去肯定审稿人的意见,然后客观的指出文章的原意,并且做出具体解释。从另一个角度来看,既然审稿人都理解错了,其他的读者更有可能,说明文章的表达还是需要改进的。建议对相关的句子重写,尽量表述得更加清晰。

在这里我们也分享一些常见的回复审稿人模板供题主参考:

当你同意审稿人意见时,可以这样写:

Thank you for your comments. We agree with your suggestion to XXX, because YYY. We have made changes to ZZZ on page/line XX as you suggested.

回复重点要包括以下几项:

你知道怎么回复审稿意见么

1.所有问题必须逐条回答。 2.尽量满足意见中需要补充的实验。3.满足不了的也不要回避,说明不能做的合理理由。 4.审稿人推荐的文献一定要引用,并讨论透彻。 以下是本人对审稿人意见的回复一例,仅供参考。续两点经验:1. 最重要的是逐条回答,即使你答不了,也要老实交代;不要太狡猾,以至于耽误事;2. 绝大部分实验是不要真追加的,除非你受到启发,而想改投另外高档杂志----因为你既然已经写成文章,从逻辑上肯定是一个完整的 “story” 了。以上指国际杂志修稿。国内杂志太多,以至于稿源吃紧,基本没有退稿,所以你怎么修都是接受。我的文章水平都不高,主要是没有明显的创新性,也很苦恼。但是除了开始几篇投在国内杂志外,其他都在国际杂志(也都是SCI)发表。以我了解的情况,我单位其他同志给国内杂志投稿,退稿的极少,只有一次被《某某科学进展》拒绝。究其原因,除了我上面说的,另外可能是我单位写稿子还是比较严肃,导师把关也比较严的缘故。自我感觉总结(不一定对):1)国内杂志审稿极慢(少数除外),但现在也有加快趋势;2)国内杂志编辑人员认真负责的人不多,稿子寄去后,少则几个月,多则一年多没有任何消息;3)国内杂志要求修改的稿子,如果你自己不修,他最后也给你发;4)国外杂志要求补充实验的,我均以解释而过关,原因见少帖)。还因为:很少杂志编辑把你的修改稿再寄给当初审稿人的,除非审稿人特别请求。编辑不一定懂你的东西,他只是看到你认真修改,回答疑问了,也就接受了(当然高档杂志可能不是这样,我的经验只限定一般杂志(影响因子1-5)。欢迎大家批评指正。我常用的回复格式:Dear reviewer:I am very grateful to your comments for the manuscript. According with your advice, we amended the relevant part in manuscript. Some of your questions were answered below.1)2)....引用审稿人推荐的文献的确是很重要的,要想办法和自己的文章有机地结合起来。至于实验大部分都可以不用补做,关键是你要让审稿人明白你的文章的重点是什么,这个实验对你要强调的重点内容不是很必要,或者你现在所用的方法已经可以达到目的就行了。最后要注意,审稿人也会犯错误,不仅仅是笔误也有专业知识上的错误,因为编辑找的审稿人未必是你这个领域的专家。只要自己是正确的就要坚持。在回复中委婉地表达一下你的意见,不过要注意商讨语气哦!我得回复格式是这样的:Dear Professor xx:Thank you very much for your letter dated xxx xx xxxx, and the referees’ reports. Based on your comment and request, we have made extensive modification on the original manuscript. Here, we attached revised manuscript in the formats of both PDF and MS word, for your approval. A document answering every question from the referees was also summarized and enclosed.A revised manuscript with the correction sections red marked was attached as the supplemental material and for easy check/editing purpose.Should you have any questions, please contact us without hesitate.然后再附上Q/A,基本上嘱条回答,写的越多越好(老师语)。结果修改一次就接收了:)我的回复,请老外帮忙修改了Dear Editor:Thank you for your kind letter of “......” on November **, 2005. We revised the manuscript in accordance with the reviewers’ comments, and carefully proof-read the manuscript to minimize typographical, grammatical, and bibliographical errors.Here below is our description on revision according to the reviewers’ comments.Part A (Reviewer 1)1. The reviewer’s comment: ......The authors’ Answer: .....2. The reviewer’s comment: ......The authors’ Answer: ...........Part B (Reviewer 2)1. The reviewer’s comment: ......The authors’ Answer: .....2. The reviewer’s comment: ......The authors’ Answer: ...........Many grammatical or typographical errors have been revised.All the lines and pages indicated above are in the revised manuscript.Thank you and all the reviewers for the kind advice.Sincerely yours,***一个回复的例子(已接收)Major comments: 1. The authors need to strengthen their results by including MMP secretion, and tran-matrigel migration by a positive control progenitor cell population i.e. enriched human CD34 cells obtained from mobilized PBL, since this is a more clinically relevant source of CD34 cells which has also been shown to secrete both MMP-9 and MMP-2 (ref. 11). CD34 enriched cells from steady state peripheral blood which also secrete MMPs are also of interest. 2. In fig 1C please specify which cell line represents MMP-negative cells. This needs to be clarified, as well as a better explanation of the method of the protocol. 3. The ELISA results are represented as "fold increase" compared to control. Instead, we suggest that standards should be used and results should be presented as absolute concentrations and only then can these results be compared to those of the zymography. 4. When discussing the results, the authors should distinguish clearly between spontaneous migration vs chemotactic migration. Furthermore, the high spontaneous migration obtained with cord blood CD34 cells should be compared to mobilized PBL CD34 enriched cells and discussed. 5. The authors claim that the clonogenic assay was performed to determine the optimum concentration for inhibition of MMP activity by phenanthroline and anti MMP-9 mAb, however they should clarify that this assay can only determine the toxicity of the inhibitors and not their optimal inhibitory concentrations.Minor comments: 1. There are many spelling and syntax errors, especially in the results and discussion, which need correction. a. Of special importance, is the percent inhibition of migration, which is described as percent of migration. i.e. pg 7:"Migration of CB CD34 was reduced to 73.3%?" Instead should read "Migration of CB CD34 was reduced by 73.3%?" b. The degree symbol needs to be added to the numbers in Materials and methods. 2. It would be preferable to combine figure 1A and B, in order to confirm the reliability of fig. 1B by a positive control (HT1080).Answer to referee 1 comment: 1. Mobilized peripheral blood is a more clinical source of CD34+ cells, so it is necessary to compare the MMP-9 secretion and trans-migration ability of CB CD34+ cells with that of mobilized PB CD34+ cells. However, we couldn't obtain enough mobilized PB to separate PB CD34+ cells and determine the MMP-9 secretion and migration ability, so we couldn’t complement the study on PB CD34+ cells in this paper. Results obtained by Janowska-Wieczorek et al found that mobilized CD34+ cells in peripheral blood express MMP-9. Furthermore, Domenech’s study showed that MMP-9 secretion is involved in G-CSF induced HPC mobilization. Their conclusions have been added in the discussion. In our present study, our central conclusion from our data is that freshly isolated CD34+ stem/progenitor cells obtained from CB produce MMP-9. 2. MMP-9 negative cell used in fig 1C was Jurkat cell. In zymographic analysis, MMP-9 was not detected in the medium conditioned by Jurkat cell. To exclude that the contaminating cells may play a role in the observed MMP-9 production, we screened the media conditioned by different proportion of CB mononuclear cells with MMP-9 negative cells by zymography. This result may be confusion. Actually, only by detecting the medium conditioned by 2X105 CB mononuclear cells (MNC)/ml (since the purities of CD34+ cell are more than 90%), it could exclude the MNC role. In the revised manuscript, we only detected MMP-9 activity and antigen level in the medium conditioned by 2X105 CB mononuclear cells (MNC)/ml. There is no MMP-9 secretion be detected in the medium conditioned by 2X105 CB MNC/ml. It excluded the possibility that the MMP-9 activity in CB CD34+ cells conditioned medium is due to the contamination by MNC. 3.In this revised paper, we have detected the MMP-9 antigen levels by using commercial specific ELISA kits (R&D System, sensitivity, 0.156ng/ml). Recombinant MMP-9 from R&D System was used as a standard. The results are expressed in the absolute concentration. The absolute concentration result has been added in the paper. As shown in Fig2, MMP-9 levels were detectable in both CB CD34+ cell conditioned medium and BM CD34+ cell conditioned medium. However, MMP-9 level was significantly higher in CB CD34+ cell conditioned medium than in BM CD34+ cell conditioned medium (0.406±0.133ng/ml versus 0.195±0.023ng/ml). Although gelatinolytic activity was not detected in media conditioned by CD34+ cells from BM, sensitivity of ELISA favors the detection of MMP-9 antigen in the BM CD34+. 4. In our study, to establish the direct link between MMP-9 and CB CD34+ cells migration, we only determined the role of MMP-9 in spontaneous migration of CB CD34+ cells, but not in chemotactic migration. Actually, regulation of hematopoietic stem cell migration, homing and anchorage of repopulation cells to the bone marrow involves a complex interplay between adhesion molecules, chemokines, cytokines and proteolytic enzymes. Results obtained by the groups of Voermans reveal that not only the spontaneous migration but also the SDF-1 induced migration of CB CD34+ cells is greatly increased in comparison to CD34+ cells from BM and peripheral blood. 5. CD34+ cells we obtained in each cord blood sample were very limited. It is not enough to screen the inhibitors concentrations to select the optimal inhibitory concentrations. In the blocking experiments, based on the concentrations used by others and the manufacturer's recommendation, we then determined the inhibitors concentrations by excluding the toxicity of the inhibitors in that concentration, which was determined by clonogenic assay.Minor comments:1.The spelling and syntax errors have been checked and corrected.2.Since the results in figure 1A and B were obtained from two separated and parallel experiments, it is not fitness to combine two figures.

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